hyperion imaging system 34 Search Results


34 cq1 confocal quantitative image cytometer  (Yokogawa Electric)
98
Yokogawa Electric 34 cq1 confocal quantitative image cytometer
34 Cq1 Confocal Quantitative Image Cytometer, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagequant las 4010 system  (GE Healthcare)
97
GE Healthcare imagequant las 4010 system
Imagequant Las 4010 System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin biotin binding fluorescence microscopy 287 cpg dna molecules ev  (Thermo Fisher)
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Thermo Fisher streptavidin biotin binding fluorescence microscopy 287 cpg dna molecules ev
Streptavidin Biotin Binding Fluorescence Microscopy 287 Cpg Dna Molecules Ev, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio observer microscope  (Carl Zeiss)
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Carl Zeiss axio observer microscope
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video camera hamamatsu orca flash 4  (Hamamatsu)
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Hamamatsu video camera hamamatsu orca flash 4
Video Camera Hamamatsu Orca Flash 4, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incucyte zoom live cell imaging system  (Sartorius AG)
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Sartorius AG incucyte zoom live cell imaging system
Incucyte Zoom Live Cell Imaging System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gadd34  (Proteintech)
96
Proteintech gadd34
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Gadd34, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imagio neo 450  (Ricoh Co Ltd)
90
Ricoh Co Ltd imagio neo 450
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Imagio Neo 450, supplied by Ricoh Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal microscope zeiss lsm780  (Carl Zeiss)
90
Carl Zeiss confocal microscope zeiss lsm780
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Confocal Microscope Zeiss Lsm780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hyperion+imaging+system+34/pmc06166812__pnas__1809039115__sapp-32-7-11?v=Carl+Zeiss
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confocal microscope zeiss lsm780 - by Bioz Stars, 2026-07
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coomassie brilliant blue  (Bio-Rad)
96
Bio-Rad coomassie brilliant blue
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Coomassie Brilliant Blue, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imager chemidoc biorad  (Bio-Rad)
96
Bio-Rad imager chemidoc biorad
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Imager Chemidoc Biorad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imager chemidoc biorad - by Bioz Stars, 2026-07
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imagelab 6 0 1  (Bio-Rad)
99
Bio-Rad imagelab 6 0 1
Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative <t>controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay,</t> normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.
Imagelab 6 0 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay, normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.

Journal: The Journal of Neuroscience

Article Title: Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse

doi: 10.1523/jneurosci.1637-16.2016

Figure Lengend Snippet: Figure 5. Perk ablation in S63del nerves reduced P-eIF2 but not CHOP activation. A, B, Western blot analysis on P28 sciatic nerves for P-eIF2 and ATF4. -tubulin (Tub) served as a loading control. n 4. C, Chop mRNA levels were measured on P28 sciatic nerves by RT-PCR TaqMan assay, normalized for 18S rRNA. S63delH (high), a different transgenic mouse that overexpresses theP0S63deltransgene(210%expression),wasusedaspositivecontrol;S63del/Chop / nerveswereusedasanegativecontrol.n6RTfromthreeindependentsetsofnervesofeachgenotype. D,CHOPproteinlevelsweredetectedbyWesternblotanalysisonP28nerves.GAPDHservedasaloadingcontrol.S63delHandS63del/Chop / sciaticnerveswereusedaspositiveandnegative controls,respectively.Onerepresentativeoutoffourindependentexperimentsisshown.E,Gadd34,Doc1,andDerlin3mRNAlevelsweremeasuredonP28sciaticnervesbyRT-PCRTaqManassay, normalizedfor18SrRNA.n3–6RTfromthreeindependentsetsofnervesforeachgenotype.F,GADD34proteinlevelsweremeasuredbyWesternblotanalysisinP28sciaticnervesandnormalized to -tubulin. n 3. *p 0.05; **p 0.01 (Student’s t test). ns, Not significant. Error bars indicate SEM.

Article Snippet: Rb pAb against calreticulin (Enzo Life Sciences, 1:1000), GRP78 (Novus Biologicals, 1:1000), GADD34 (Proteintech, 1:500), and rat mAb GRP94 (Abcam, 1:1000) were detected with the Odyssey CLx infrared imaging system (Li-Cor).

Techniques: Activation Assay, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction, TaqMan Assay, Transgenic Assay, Expressing